CNRS research director
Université Paris-Saclay

Biography

My research aims to address fundamental issues in self-organizing biological matter at the microscopic scale using experimental and theoretical concepts derived from soft condensed matter physics. In particular, I focus on the high-resolution structure of ordered and weakly disordered systems, as well as on the nonequilibrium dynamics of assembly and self-organization phenomena. I exploit state-of-the-art techniques including cryotransmission electron microscopy and time-resolved radiation scattering with large-scale facilies, and develop models that account for the observed complex behaviors.

Interests

  • Biological self-assembly and self-organization
  • Viruses
  • DNA condensates
  • Small-angle scattering
  • Microfluidics

Education

  • Accreditation to supervise research (2013), Université Paris-Sud
  • PhD in physics (2002), Université Aix-Marseille I
  • MSc. in physics (1998), Université Paris-Sud
  • MEng. in electrical engineering (1998), Ecole Supérieure d’Electricité (Supélec)

Statistical data analysis

Master 2 Systèmes Biologiques et Concepts Physiques
Doctoral school EDPIF Physique en Ile-de-France

Five or ten 3-hours sessions each split into half lecture and half practicals in Python.

Research in physics requires the acquisition, analysis and interpretation of experimental measurements in order to verify an hypothesis and/or devise a theoretical model. The great variability inherent to many physical systems makes it essential to take into account the uncertainties and to use powerful statistical tools available today via Python. Each session consists of a first theoretical part that presents the fundamental concepts of statistical data analysis, illustrated in the second part of the course by practical work in Python through concrete situations encountered in physics and biophysics. The main topics covered include a reminder of probabilities and distributions, statistical tests (chi2, Student, p-value), handling experimental uncertainties (confidence intervals), linear and non-linear adjustments (least squares, covariance matrix, correlations), and some advanced notions (bayesian inference, statistical learning).

Small-angle X-ray scattering and colloidal systems

Ecole Supérieure de Physique et de Chimie Industrielle de la Ville de Paris (ESPCI)

Two sessions of 2 hours each.

This course is an introduction to small-angle X-ray scattering applied to dilute and crystallized colloidal systems. It gives the fundamentals of X-ray scattering by one electron, then the interferences obtained in the presence of two electrons and the generalization with a continuous electron density. The main results for a solution of spherical particles are deduced analytically. Actual scattering data of colloidal suspensions are analyzed thus providing the methodology for characterizing nanoparticles and liquid crystals at the microscopic scale.

Virus self-assembly in complex environments

Viruses are amazing biological agents in which hundreds of molecular blocks integrate with atomic precision into the final structure. Their regularity is all the more remarkable because for many viruses it occurs spontaneously, in an efficient self-assembly process, whether in the host cell or in a test tube. Many viruses are assembled in a crowded intracellular environment, with a low error rate, despite numerous nonspecific interactions with cellular components. Our objective is to decipher the self-assembly pathways of icosahedral viruses in complex, cell-like environments, by setting up a cross-disciplinary strategy that combines experimental physics and quantitative biology with an advanced soft-matter theoretical framework.


Out-of-equilibrium cellular droplets

Living cells are organized into distinct subcompartments to facilitate spatiotemporal regulation of biological reactions. While this organization is traditionally thought to rely on membrane-delimited organelles, such as the nucleus and mitochondria, it also involves membrane-less assemblies of proteins and nucleic acids that are kept together only by weak, dynamical interactions between their components. In the last years groundbreaking studies suggested that these cellular assemblies have liquid-like physical properties and are formed via liquid-liquid phase separation (LLPS) processes. We plan to rely on state-of-the-art techniques in soft matter physics and on an advanced molecular modeling framework for unveiling general features of cellular LLPS in equilibrium and nonequilibrium conditions
and linking them back to their molecular origin.


Marangoni flows

The Marangoni effect is a spectacular manifestation of the interfacial forces driven by surfactants at the air-water interface. It stems from the gradients of surface tension due to the inhomogeneity of the surfactant density across the interface, which subsequently induce the
motion of water over a thin layer. This project aims at elucidating the flows generated in the bulk by visualizing them with a fluorescent probe.

Ruszkowski, M., Strugala, A., Indyka, P., Tresset, G., Figlerowicz, M., & Urbanowicz, A. (2022). Cryo-EM reconstructions of BMV-derived virus-like particles reveal assembly defects in the icosahedral lattice structure. Nanoscale, 14(8), 3224–3233. https://doi.org/10.1039/D1NR05650F
Marichal, L., Gargowitsch, L., Rubim, R. L., Sizun, C., Kra, K., Bressanelli, S., Dong, Y., Panahandeh, S., Zandi, R., & Tresset, G. (2021). Relationships between RNA topology and nucleocapsid structure in a model icosahedral virus. Biophysical Journal, 120(18), 3925–3936. https://doi.org/10.1016/j.bpj.2021.08.021
Du, H., de Oliveira, F. A., Albuquerque, L. J. C., Tresset, G., Pavlova, E., Huin, C., Guégan, P., & Giacomelli, F. C. (2020). Polyglycidol-Stabilized Nanoparticles as a Promising Alternative to Nanoparticle PEGylation: Polymer Synthesis and Protein Fouling Considerations. Langmuir, 36(5), 1266–1278. https://doi.org/10.1021/acs.langmuir.9b03687
Chevreuil, M., Lecoq, L., Wang, S., Gargowitsch, L., Nhiri, N., Jacquet, E., Zinn, T., Fieulaine, S., Bressanelli, S., & Tresset, G. (2020). Nonsymmetrical Dynamics of the HBV Capsid Assembly and Disassembly Evidenced by Their Transient Species. The Journal of Physical Chemistry B, 124(45), 9987–9995. https://doi.org/10.1021/acs.jpcb.0c05024
Tresset, G. (2013). Compartimentation microscopique: depuis kes microchambres femtolitriques jusqu’aux particules pseudo-virales [HDR]. Université Paris-Sud.
Ni, M., Tresset, G., Iliescu, C., & Hauser, C. A. E. (n.d.). Ultrashort Peptide Theranostic Nanoparticles by Microfluidic-Assisted Rapid Solvent Exchange [IEEE].
Ni, M., Tresset, G., Iliescu, C., & Hauser, C. A. E. (2020). Ultrashort Peptide Theranostic Nanoparticles by Microfluidic-Assisted Rapid Solvent Exchange. IEEE Transactions on NanoBioscience, 19(4), 627–632. https://doi.org/10.1109/TNB.2020.3007103
Panahandeh, S., Li, S., Marichal, L., Leite Rubim, R., Tresset, G., & Zandi, R. (2020). How a Virus Circumvents Energy Barriers to Form Symmetric Shells. ACS Nano, 14(3), 3170–3180. https://doi.org/10.1021/acsnano.9b08354
Tresset, G., Castelnovo, M., & Leforestier, A. (2017). Assemblage et désassemblage des virus : mode d’emploi. Reflets de La Physique, 52, 22–26. https://doi.org/10.1051/refdp/201752022
Illy, N., Corcé, V., Zimbron, J., Molinié, V., Labourel, M., Tresset, G., Degrouard, J., Salmain, M., & Guégan, P. (2019). pH‐Sensitive Poly(ethylene glycol)/Poly(ethoxyethyl glycidyl ether) Block Copolymers: Synthesis, Characterization, Encapsulation, and Delivery of a Hydrophobic Drug. Macromolecular Chemistry and Physics, 1900210. https://doi.org/10.1002/macp.201900210
Chevreuil, M., Fieulaine, S., Poncet, L., Perronet, K., Zinn, T., Jacquet, E., Nhiri, N., Bressanelli, S., & Tresset, G. (2019). Characterization of the Assembly and Disassembly of Capsid Proteins Derived from Hepatitis B Virus. Biophysical Journal, 116(3), 159a. https://doi.org/10.1016/j.bpj.2018.11.879
Gomez, J.-P., Tresset, G., Pichon, C., & Midoux, P. (2019). Improved histidinylated lPEI polyplexes for skeletal muscle cells transfection. International Journal of Pharmaceutics, 559, 58–67. https://doi.org/10.1016/j.ijpharm.2019.01.003
Tresset, G. (2019, January). Impact of RNA on the assembly dynamics of icosahedral and tubular viruses. Physical Virology Gordon Research Conference, Ventura (USA).
Chevreuil, M., Law-Hine, D., Chen, J., Bressanelli, S., Combet, S., Constantin, D., Degrouard, J., Möller, J., Zeghal, M., & Tresset, G. (2018). Nonequilibrium Self-Assembly Dynamics of Icosahedral Viral Capsids Packaging Genome. Biophysical Journal, 114(3), 60a. https://doi.org/10.1016/j.bpj.2017.11.378
Burke, A., Chevreuil, M., Paris, A., de La Grange, V., Goldmann, C., Pérez, J., Constantin, D., & Tresset, G. (2018). Nanoparticle-Templated Self-Assembly of Viral Capsids Probed by Time-Resolved Absorbance Spectroscopy and X-Ray Scattering. Physical Review Applied, 10(5), 054065–054075. https://doi.org/10.1103/PhysRevApplied.10.054065
Chen, J., Lansac, Y., & Tresset, G. (2018). Interactions between the Molecular Components of the Cowpea Chlorotic Mottle Virus Investigated by Molecular Dynamics Simulations. The Journal of Physical Chemistry B, 122(41), 9490–9498. https://doi.org/10.1021/acs.jpcb.8b08026
Tresset, G. (2018, Août). Microfluidics-directed self-assembly of DNA-based nanoparticles. International Microfluidics Congress, San Diego (USA).
Chevreuil, M., Law-Hine, D., Chen, J., Bressanelli, S., Combet, S., Constantin, D., Degrouard, J., Möller, J., Zeghal, M., & Tresset, G. (2018). Nonequilibrium self-assembly dynamics of icosahedral viral capsids packaging genome or polyelectrolyte. Nature Communications, 9(1). https://doi.org/10.1038/s41467-018-05426-8
Tresset, G., Chen, J., Chevreuil, M., Nhiri, N., Jacquet, E., & Lansac, Y. (2017). Two-Dimensional Phase Transition of Viral Capsid Gives Insights into Subunit Interactions. Physical Review Applied, 7(1), 014005. https://doi.org/10.1103/PhysRevApplied.7.014005
Tresset, G., Marculescu, C., Salonen, A., Ni, M., & Iliescu, C. (2013). Fine Control Over the Size of Surfactant–Polyelectrolyte Nanoparticles by Hydrodynamic Flow Focusing. Analytical Chemistry, 85(12), 5850–5856. https://doi.org/10.1021/ac4006155
Maury, B., Gonçalves, C., Tresset, G., Zeghal, M., Cheradame, H., Guégan, P., Pichon, C., & Midoux, P. (2014). Influence of pDNA availability on transfection efficiency of polyplexes in non-proliferative cells. Biomaterials, 35(22), 5977–5985. https://doi.org/10.1016/j.biomaterials.2014.04.007
Tresset, G., Tatou, M., Le Cœur, C., Zeghal, M., Bailleux, V., Lecchi, A., Brach, K., Klekotko, M., & Porcar, L. (2014). Weighing Polyelectrolytes Packaged in Viruslike Particles. Physical Review Letters, 113(12), 128305. https://doi.org/10.1103/PhysRevLett.113.128305
Tresset, G., Le Coeur, C., Bryche, J.-F., Tatou, M., Zeghal, M., Charpilienne, A., Poncet, D., Constantin, D., & Bressanelli, S. (2013). Norovirus Capsid Proteins Self-Assemble through Biphasic Kinetics via Long-Lived Stave-like Intermediates. Journal of the American Chemical Society, 135(41), 15373–15381. https://doi.org/10.1021/ja403550f
Tresset, G. (2018, Mai). Genome- and Polymer-filled Icosahedral Viruses. Journées de la Diffusion Neutronique, Roquebrune-sur-Argens (France).
Tresset, G. (2018, Avril). Understanding Virus Self-Assembly from X-ray and Neutron Scattering. 7th Spring World Congress on Engineering and Technology, Guilin (Chine).
Tresset, G. (2015). The self-assembly of icosahedral capsids probed by X rays and neutrons. Journée Biophysique Université Paris-Saclay, Gif-sur-Yvette, France.
Tresset, G. (2017, juin). Nonequilibrium Dynamics of RNA Packaging in Icosahedral Viral Capsids. ICTP Workshop on Physical Virology, Trieste, Italie.
Tresset, G. (2016, September). Self-assembly/Disassembly Dynamic Pathways of an Icosahedral Viral Capsid. Proc. 4th International Soft Matter Conference, Grenoble, (France).
Tresset, G. (2016, September). Microfluidics-Directed Self-Assembly of DNA-Based Nanoparticles. Proc. 4th International Soft Matter Conference, Grenoble, (France).
Tresset, G. (2015, Février). Assembly and Disassembly Kinetics of Virus-Like Particles Probed by Time-Resolved Small-Angle X-Ray Scattering. Proc. 10th SOLEIL Users’ Meeting, Palaiseau (France).
Tresset, G. (2014, Août). Weighing Polyelectrolytes Packaged in Virus-Like Particles. Proc. Mathematical Virology Conference, York (UK).
Tresset, G. (2013, October). Norovirus capsid proteins self-assemble through biphasic kinetics via long-lived stave-like intermediates. Proc. 23rd Phage/Virus Assembly, Lake Arrowhead (USA).
Iliescu, C., & Tresset, G. (2015). Microfluidics-Driven Strategy for Size-Controlled DNA Compaction by Slow Diffusion through Water Stream. Chemistry of Materials, 27(24), 8193–8197. https://doi.org/10.1021/acs.chemmater.5b04129
Law-Hine, D., Sahoo, A. K., Bailleux, V., Zeghal, M., Prevost, S., Maiti, P. K., Bressanelli, S., Constantin, D., & Tresset, G. (2015). Reconstruction of the Disassembly Pathway of an Icosahedral Viral Capsid and Shape Determination of Two Successive Intermediates. The Journal of Physical Chemistry Letters, 6(17), 3471–3476. https://doi.org/10.1021/acs.jpclett.5b01478
Chen, J., Chevreuil, M., Combet, S., Lansac, Y., & Tresset, G. (2017). Investigating the thermal dissociation of viral capsid by lattice model. Journal of Physics: Condensed Matter, 29(47), 474001. https://doi.org/10.1088/1361-648X/aa8d88
Tresset, G., & Iliescu, C. (2016). Microfluidics-Directed Self-Assembly of DNA-Based Nanoparticles. INFORMACIJE MIDEM-JOURNAL OF MICROELECTRONICS ELECTRONIC COMPONENTS AND MATERIALS, Vol. 46, No. 4(2016), 183 – 189.
Ni, M., Tresset, G., & Iliescu, C. (2017). Self-assembled polysulfone nanoparticles using microfluidic chip. Sensors and Actuators B: Chemical, 252, 458–462. https://doi.org/10.1016/j.snb.2017.06.028
Tresset, G. (2016, September). The Assembly and Disassembly Pathways of Viral Nanoshells Probed by X-Ray and Neutron Scattering. CMD26 Physics of Protein Nanoshells, Groningen, (Pays-Bas).
Tresset, G. (2016, October). Microfluidics-Directed Self-Assembly of DNA-Based Nanoparticles. 52nd International Conference on Microelectronics, Devices and Materials, Ankaran, (Slovénie).
Law-Hine, D., Zeghal, M., Bressanelli, S., Constantin, D., & Tresset, G. (2016). Identification of a major intermediate along the self-assembly pathway of an icosahedral viral capsid by using an analytical model of a spherical patch. Soft Matter, 12(32), 6728–6736. https://doi.org/10.1039/C6SM01060A
Tresset, G. (2015, October). Biomimetic assemblies processed by microfluidics. International Semiconductor Conference, Sinaia (Roumanie).
Tresset, G. (2016, Juillet). What can we learn from the disassembly of icosahedral viral capsids? FASEB SRC Virus Structure and Assembly, Steamboat Springs, USA.
Iliescu, C., Tresset, G., Yu, L., & Xu, G. (2010). 3d Dielectrophoretic chips : trapping and separation of cell populations. J. of Information Science and Technology, 13, 49–64.
Tresset, G., Iliescu, C., & Xu, G. (2009). Cell sorting in a dielectrophoretic device with asimetric electrodes. IEEE, 1 et 2, 297–300.
Tresset, G., Iliescu, C., & Xu, G. (2009). Dielectrophoretic field-flow method for separating particle populations in a chip with asymmetric electrodes. Biomicrofluidics, 3, 044104.
Tresset, G., & Lansac, Y. (2011). Long-range architecture of single lipid-based complex nanoparticles with local hexagonal packing. J. Phys. Chem. C, 2, 41–46.
Tresset, G. (2009). The multiple faces of self-assembled lipidic systems. PMC Biophysics, 2, 3.
Tresset, G. (2010). Live/dead cell assay based on dielectrophoresis-on-a-chip. University  Politehnica of Bucharest Scientific Bulletin-Séries A  Appl. Math. and Phys., 72, 33–44.
Tresset, G. (2010). Vers les scénarios avancés dans le tokamak européen jet. Editions Universitaires Européennes.
Iliescu, C., Xu, G., Tong, W. H., Yu, F., Bălan, C. M., Tresset, G., & Yu, H. (2015). Cell patterning using a dielectrophoretic–hydrodynamic trap. Microfluidics and Nanofluidics, 19(2), 363–373. https://doi.org/10.1007/s10404-015-1568-2

Contact

Laboratoire de Physique des Solides, Université Paris-Saclay, CNRS (Orsay, France)

Jan 2021 – present: Deputy director
Oct 2019 – present: CNRS research director
Jul 2019 – Dec 2020: Head of the “Self-Assembled Biological Objects” team
Oct 2008 – Oct 2019: CNRS research associate

Institute of Bioengineering and Nanotechnology, A*STAR (Republic of Singapore)

Jan 2005 – Sep 2008: Research scientist       

Laboratory for Integrated Micro Mechatronic Systems, The University of Tokyo (Japan)

Nov 2002 – Dec 2004: Postdoctoral fellow

Département de Recherches pour la Fusion Contrôlée, CEA (Saint-Paul-lèz-Durance, France)

Oct 1999 – Sep 2002: Doctoral candidate